<span style="font-size:13.0pt;mso-bidi-font-size: 11.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US; mso-bidi-language:HI" lang="EN-GB">Antimicrobial efficacy of <i style="mso-bidi-font-style: normal">Bridelia retusa</i> (Linn.) Spreng. and <i style="mso-bidi-font-style: normal">Asclepias curassavica</i> Linn.</span>

Abstract

The <i style="mso-bidi-font-style:normal">in vitro</i> bioassay of the bark extracts of <i style="mso-bidi-font-style:normal">Bridelia retusa</i> Spreng. and root extracts of <i style="mso-bidi-font-style:normal">Asclepias curassavica</i> Linn. was done by cold percolation and Soxhlet method against four bacterial species, viz.<i style="mso-bidi-font-style:normal"> Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa</i> and <i style="mso-bidi-font-style:normal">Proteus vulgaris</i> and two fungal species <i style="mso-bidi-font-style:normal">Candida albicans</i> and <i style="mso-bidi-font-style:normal">Aspergillus niger</i>. The MIC and MBC values for the extracts obtained by both the methods were tested separately. The acetone extract of <i style="mso-bidi-font-style:normal">B. retusa</i> showed MIC values ranging from 25, 42.5 to 85 mg/mL against the tested strains where as the bactericidal and fungicidal concentrations were found to be ≥170 mg/mL. The MIC value for root extract of <i style="mso-bidi-font-style: normal">A. curassavica</i> was 3.06 mg/mL and the bactericidal concentration was found to be 100 mg/mL. The present work is an attempt to justify that the method of extraction influences the efficacy of the plant extracts.