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http://nopr.niscpr.res.in/handle/123456789/15691| Title: | Bioconversion producing D-p-hydroxyphenylglycine from DL-p-hydroxyphenylhydantoin by Pseudomonas putida in aqueous two-phase system |
| Authors: | Qian, Junqing Chen, Changchun Liu, Min Lv, Binfeng |
| Keywords: | Aqueous two-phase system;D-hydantoinase;DL-p-hydroxyphenylhydantoin;D-p-hydroxyphenylglycine;N-carbamoylase;N-carbamoyl-D-p-hydroxyphenylglycine |
| Issue Date: | Oct-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The whole cell enzymatic conversion of DL-p-hydroxyphenylhydantoin (DL-p-HPH) to D-p-hydroxyphenylglycine (D-p-HPG) was carried out in aqueous two-phase system by Pseudomonas putida AS 11820 with D-hydantoinase (D-Hase) and N-carbamoylase (N-Case). The optimum aqueous two-phase system, having partition coefficient of DL-p-HPH (KDL-p-HPH), N-carbamoyl-D-p-hydroxyphenylglycine (CpHPG, KCpHPG) and D-p-HPG (KD-p-HPG) to be 28.6, 1.45 and 1.25, respectively, consisted of poly ethylene glycol (PEG) 1500 (15%, w/v) and sodium sulphate (15%, w/v). When the concentration of DL-p-HPH as substrate was 50 mM and the concentration of the rest cell was 0.10 g/mL at pH 9.9 and 28oC, the highest production yield (30.25 mM) of D-p-HPG was achieved. The activity of D-Hase remained 71% after resting cells were retained in the aqueous two-phase system for 40 h. |
| Page(s): | 445-452 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.11(4) [October 2012] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 11(4) 445-452.pdf | 316.77 kB | Adobe PDF | View/Open |
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