Chromosomal location of non-hypersensitive leaf rust resistance genes in bread wheat cultivar PBW65 using microsatellite markers

Abstract

<span style="letter-spacing:-.1pt;mso-bidi-font-style: italic" lang="EN-GB">Microsatellite or simple sequence repeat (SSRs) markers have been powerful tool for genetic mapping in wheat. Indian bread wheat (<i style="mso-bidi-font-style:normal">Triticum aestivum </i><span style="mso-bidi-font-style:italic">L<i style="mso-bidi-font-style:normal">.</i>) cultivar PBW65 has shown significant level of resistance to most virulent race 77-5 of leaf rust (<i style="mso-bidi-font-style:normal">Puccinia triticina</i>). It has been indicated that PBW65 expresses non-hypersensitive type of resistance against race 77-5. F₂ and F₃ crossing of PBW65 with WL711, a leaf rust susceptible wheat cultivar, and allelic tests with such already known genes (present in cultivars RL 6058 and HD 2009) revealed that cultivar PBW65 could be a potential source of novel non-hypersensitive leaf rust resistance genes. So far, only non-hypersensitive leaf rust resistance gene <i style="mso-bidi-font-style:normal">Lr34</i> was found to be effective under Indian conditions. <span style="mso-bidi-font-weight:bold">Attempts to locate such durable leaf rust resistance genes in PBW65 through microsatellite markers showed 2B, 2D and 3D as critical chromosomes for PBW65. The primer <i style="mso-bidi-font-style:normal">Xgwm341</i> (3D) was found located 41.5 cM away from gene <i style="mso-bidi-font-style:normal">LrPBW1</i> in PBW65.

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