Effect of solvents on the hydrolysis of casein catalysed by pepsin-A kinetic study

Abstract

It is observed that pepsin shows increased catalytic activity in

aquo-alcoholic media compared to aqueous media. The maximum

activity of pepsin has been recorded in the 20% to 40% range of

<span style="font-size:14.0pt;mso-bidi-font-style:italic">0.2<i>M </i>of aquo-alcoholic media. Kinetics of pepsin catalysed hydrolysis

<span style="font-size:14.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-ansi-language:en-us;mso-fareast-language:en-us;="" mso-bidi-language:ar-sa"="">of casein has been investigated in different alcohols viz., methanol, ethanol, <i style="mso-bidi-font-style:normal">n</i>-propanol, isopropanol and <i style="mso-bidi-font-style:normal">t</i>-butanol. At <span style="mso-bidi-font-style:italic">0.2<i>M </i>alcohol concentration thermal stability and activity of pepsin increases from primary to tertiary alcohols. The activity decreases and thermal stability increases with further increase in alcohol concentration.</span></span></span>