Oxidation and estimation of bilirubin by using carbon microelectrode

Abstract

<span style="font-size:14.5pt;mso-bidi-font-size:8.5pt">Bilirubin is one of the major bile pigment, clinically and biologically important among the bile pigments present in the mammal. When the concentration or bilirubin exeeds the allowable limit, it leads to a number or disorders and ailments in the human beings including jaundice. Bi1irubin is present in the reduced form which is highly toxic whereas its oxidized form biliverdin is nontoxic. Hence the oxidation and estimation of bilirubin is essential in the clinical arena. A<span style="font-size:13.5pt;mso-bidi-font-size:7.5pt;font-family:Arial"> <span style="font-size:14.5pt;mso-bidi-font-size:8.5pt">differential pulse voltammetric method has been recommended for<span style="font-size:13.0pt;mso-bidi-font-size: 7.0pt"> <span style="font-size:14.5pt;mso-bidi-font-size:8.5pt">the estimation of<span style="font-size:13.0pt;mso-bidi-font-size:7.0pt"> <span style="font-size:14.5pt;mso-bidi-font-size:8.5pt">bilirubin with a bare carbon microelectrode in the aqueous medium and indirect oxidation of bilirubin to biliverdin. The oxidation has been carried out using <i style="mso-bidi-font-style: normal">in situ </i>electrogenerated hydrogen peroxide produced from the continuous reduction of oxygen at its reduction potential. Estimation of bilirubin has been done using its reduction peak observed at around <span style="font-size:14.0pt;mso-bidi-font-size:8.0pt">-1.1<span style="font-size:14.5pt;mso-bidi-font-size:8.5pt">V. The kinetic studies of indirect oxidation of bilirubin has been followed spectrophotometrically in the visible region.<span style="font-size:16.0pt;mso-bidi-font-size:10.0pt"> </span></span></span></span></span></span></span></span></span></span>