Solid phase synthesis of diazepam binding inhibitor fragments 32-38 and 45-50 on a flexible butanediol dimethacrylate crosslinked polystyrene support

Abstract

<span style="font-size:12.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-ansi-language:="" en-in;mso-fareast-language:en-in;mso-bidi-language:ar-sa"="" lang="EN-IN">Two short sequences of the neuropeptide Diazepam Binding Inhibitor (DBI) KQATVGD (32-38) and GLLDLK (45-50) have been synthesized on a butanediol dimethacrylate (BDDMA) crosslinked polystyrene support by the solid phase technique. The BDDMA crosslinked polystyrene support is prepared by the radical initiated suspension polymerization of the monomers. These supports are found to possess very good mechanical and physical properties. The solvation properties of the new polymer support are found to be superior to the commonly employed styrene-DYB resins. A 2 mole percent crosslinked polymer support is found to be ideal for peptide synthesis. The BDDMA-PS resin is functionalised by chloromcthylation. Polymer support with a chlorine capacity 1.8 mmol/g is used for peptide synthesis. The first amino acid is appended to the polymer support by Gisin's cesium salt method. The syntheses of the target peptides are carried out by the usual solid phase strategy using Boc amino acids. After synthesis the peptides are cleaved from the support using trifluoroacetic acid . The peptides are obtained in high yield and the purity of the peptides are checked by TLC and HPLC.</span>