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http://nopr.niscpr.res.in/handle/123456789/16537| Title: | DNA cassette for detection and IPR protection of transgenic Cyanobacteria and Rhizobium strains |
| Authors: | Biate, David L Misra, Arvind K |
| Keywords: | Cyanobacteria;DNA cassette;Frankia;Intellectual property rights (IPR);Rhizobium;Transgenics |
| Issue Date: | Jan-2013 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | We report the potential utility of using partial 16S ribosomal RNA sequence of Frankia for tagging transgenic Cyanobacteria and Rhizobium strains for addressing intellectual property right (IPR) issues. We have introduced restriction sites in the primers for securing amplification of the distal region of 16S rDNA of Frankia. The amplicon then can be included in the construct for genetic engineering of cyanobacteria or rhizobium and shall remain embedded as a signature for easy identification of the engineered strains. This will help in protecting IPR issues with respect to genetically modified organisms. For this purpose, the Frankia 16S rDNA sequence was amplified using Frankia specific primers targeting ~500 bp at the 3′ end containing helices 34 and 44 that play important role in translation. The 3′ end of the amplified 16S rDNA sequence also contains the Shine-Dalgarno complementary sequence, which is essential for the initiation of translation. Multiple alignments of the 16S rDNA sequences of Frankia sp. were therefore carried out with 84 corresponding 16S rDNA sequences of cyanobacteria and Rhizobium to determine if there was/were any mutation(s) within these regions that could affect translation in the host. The analysis revealed that these regions are highly conserved across the three different bacterial species. Therefore, event of chance recombination between the introduced DNA sequence and the host’s gene is not likely to have any adverse effect on the modified organism. It is also a non-expressing sequence, giving an added ecological advantage to the target host. |
| Page(s): | 103-108 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.12(1) [January 2013] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 12(1) 103-108.pdf | 183.46 kB | Adobe PDF | View/Open |
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