<span style="font-size:12.0pt;line-height: 115%;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-IN">In <i>vitro</i> mass multiplication of <i>Ophiorrhiza mungo</i> Linn.</span>

Abstract

<span style="font-size:12.0pt;line-height: 115%;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">A protocol for in <i>vitro</i> mass multiplication of plants through seedling (shoot) cultures was established for <i>Ophiorrhiza</i> <i>mungo.</i> Maximum number of adventitious shoots per shoot culture (10.4 ± 1.72) was initiated on MS solid medium supplemented with BAP (2.22μ<i>M</i>) after 3 weeks. Shoots were further multiplied (12.8 ± 2.8) through subculture of intact shoots and reculture of nodal segments of aseptic shoots (6.5±0.94) in MS solid medium containing BAP (0.89μ<i>M</i>). Shoot elongation (1.27 ± 0.1 2 cm) was achieved in the medium containing GA₃ (1.44μ<i>M</i>) in two weeks. Rooting was favoured in basal agar medium supplemented with IBA (12.3μ<i>M</i>) plus NAA ( 1.07μ<i>M</i>). The plants were successfully established (100%) in the pots containing sand and top soil (1:1) mixture in a period of two weeks.</span>