Differential organization of a LINE-1 family in Indian pygmy field mice

Abstract

<span style="font-size:12.0pt;mso-bidi-font-size: 14.0pt;line-height:115%;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-ansi-language:en-in;mso-fareast-language:en-in;="" mso-bidi-language:hi"="" lang="EN-IN">Southern blot hybridization analysis of genomic DNAs digested with restriction endonuclease EcoR I and Ava II from <i>Mus musculus domesticus, Mus booduga</i> and <i>Mus terricolor</i> with a cloned repetitive DNA fragment of <i>Mus booduga</i> as a probe showed difference in restriction pattern of this DNA in these three species. Further Southern analysis of the BamH I digested genomic DNAs from these species hybridized with cloned DNA fragment as a probe and sequencing of the cloned DNA revealed that this 252 bp cloned DNA fragment is a part of BamHI repeat element of genus <i>Mus</i> and is 87 % homologous to the contiguous portion of the <i>Mus musculus domesticus</i> LlNE- 1 element. The species specific fragment pattern generated by different restriction endonucleases using this DNA as a probe revealed difference in the organization of LlNE- 1 repetitive element in the three species of genus <i>Mus.</i></span>