<span style="font-size:12.0pt;mso-bidi-font-size: 14.0pt;line-height:115%;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-ansi-language:EN-IN;mso-fareast-language:EN-IN; mso-bidi-language:HI" lang="EN-IN">Production of thiophenes from callus cultures of <i>Tagetes patula</i> L. and its mosquito larvicidal activity</span>

Abstract

<span style="font-size:12.0pt;mso-bidi-font-size: 14.0pt;line-height:115%;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-ansi-language:en-in;mso-fareast-language:en-in;="" mso-bidi-language:hi"="" lang="EN-IN">Calli were initiated from leaf tissues of <i>T. patula</i>, on Murashige and Skoog's medium supplemented with 2-4 dichlorophenoxacetic acid (2,4-D) and kinetin (Kn). The maximum accumulation of biomass was recorded on 6th week at 1.11 g. Dry wt/culture. Maximum thiophene content was recorded on 4th week at 0.008 % on dry wt basis in callus cultures of <i>T. patula</i> subjected to nutrient stress. Nitrogen stress induced 3-fold increase in thiophene production level in six weeks (0.024 % on dry weight basis). The best hormonal supplementation required for thiophenes production was found to be 2,4-D (2.0 mgL^-1) and kinetin (2.0 mg L^-1) . The thiophenes produced in callus cultures of <i>T. patula</i> showed larvicidal effect against mosquito larvae.</span>