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http://nopr.niscpr.res.in/handle/123456789/17012| Title: | Establishment of embryonic cultures and somatic embryogenesis in callus culture of guggul-Commiphora wightii (Arnott.) Bhandari |
| Authors: | Kumar, Sandeep Suri, S S Sonie, K C Ramawat, K G |
| Issue Date: | Jan-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Somatic embryogenesis in callus cultures of Commiphora wigitii (Arnott.) Bhandari was achieved. Though the frequency of explants producing embryonic culture was low, immature zygotic embryos were the only suitable explants to produce embryonic callus after reciprocal transfers on media containing 2,4,5-trichlorophenoxy acetic acid (0. 1 mgl-1) and kinetin(0.1mgl-1 ) or devoid of growth regulators. All other media failed to produce embryonic callus. Embryonic cells were small, densely filled with cytoplasm and isodiametric as compared to non-embryonic cells, which were large, elongated and vacuolated. Maximum growth of embryonic callus was recorded on modified MS medium (MS-2 medium) supplemented with BA (0.25 mgl-1) and IBA (0.1 mgl-1). MS-2 salts supported higher growth of callus as compared to tissues grown on B5 medium containing same concentrations of plant growth regulators. Exogenous medium nutrients had no effect on somatic embryo development whereas plant growth regulators had little effect. Asynchronously growing embryos formed plantlets regularly which were successfully transferred to the field conditions. |
| Page(s): | 69-77 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.41(01) [January 2003] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 41(1) 69-77.pdf | 1.88 MB | Adobe PDF | View/Open |
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