<span style="font-size:12.0pt;mso-bidi-font-size: 14.0pt;line-height:115%;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-ansi-language:EN-IN;mso-fareast-language:EN-IN; mso-bidi-language:HI" lang="EN-IN">Effect of phenol on ultra structure and plasmid DNA of <i>Xanthomonas oryzae </i><span style="mso-bidi-font-style:italic">pv<i>. oryzae</i></span></span>

Abstract

<span style="font-size:12.0pt;mso-bidi-font-size: 14.0pt;line-height:115%;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-ansi-language:en-in;mso-fareast-language:en-in;="" mso-bidi-language:hi"="" lang="EN-IN">Most phenolic substances of plant origin are toxic to microorganisms and they confer some degree of protection to plants against phytopathogens. <i>Xanthomonas oryzae pv. oryzae</i>, bacterial blight pathogen of rice (<i>Oryza sativa</i>) was treated with phenol (monohydroxy benzene) and its effects on the morphology and cytological changes of the bacterium were studied. Total lysis of cells occurred with 5 m<i>M</i> cone of phenol while at 2 m<i>M</i> cone, the cell walls became rough and cell contents started shrinking. Plasmids isolated from both treated (2 m<i>M</i>) and control cells did not show any marked difference under electron mi croscope except that they differed in their quantity and might influence pathogenicity.</span>