Association of cucumovirus and potyvirus with betelvine <i>(Piper </i><i>betl</i><i>e </i><i>L.)</i> <span style="font-size:14.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";color:#1C1C1C;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI">as evidenced by ELISA and RT-PCR</span>

Abstract

An attempt was made to detect various viruses of <i>Pip</i><i>e</i><i>r </i><i>bet</i><i>l</i><i>e </i>grown at Mahoba and Banthara in India. DAC-ELISA and RT-PCR tests were performed in leaf sap samples of betelvine for detection of a cucumovirus <i>(cucumber mosaic virus</i><i>)</i>and potyvirus <i>(</i><i>B</i><i>ea</i><i>n </i><i>ye</i><i>ll</i><i>ow </i><i>m</i><i>osa</i><i>i</i><i>c </i><i>viru</i><i>s) </i>using specific antibodies and universal primers of respective viruses. DAC-E LISA could detect only CMV. However, RT-PCR detected both cucumovirus and potyvirus infection in betelvine samples. Association of CMV with betelvine was observed for the first time in the present study.