Please use this identifier to cite or link to this item:
Title: Curing of symbiotic plasmid of Mesorhizobium huakuii subsp. rengei isolated from Astragalus sinicus
Authors: Balachandar, D
Kannaiyan, S
Ono, H
Murooka, Y
Keywords: Astragalus sinicus; Mesorhizobium huakuii;Symbiotic plasmid
Issue Date: Aug-2003
Publisher: NISCAIR-CSIR, India
Abstract: Astragalus sinicus (Chinese Milk vetch), a green manure leguminous plant, harbors Mesorhizobium huakuii subsp. rengei strain B3 in the root nodules. The visualization of symbiotic plasmid of strain B3 showed the presence of one sym plasmid of about 425 kbp. Curing of sym plasmid by temperature and acrydine orange was studied. Growing rhizobial cells at high temperature (37°C) or treating the cells with acrydine orange at 50 mg/I eliminated sym plasmid of M. huakuii strain B3, which was confirmed by sym plasmid visualization and plant infection test of cured strains.
Page(s): 912-914
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.41(08) [August 2003]

Files in This Item:
File Description SizeFormat 
IJEB 41(8) 912-914.pdf702.24 kBAdobe PDFView/Open

Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.