Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/17129| Title: | Curing of symbiotic plasmid of Mesorhizobium huakuii subsp. rengei isolated from Astragalus sinicus |
| Authors: | Balachandar, D Kannaiyan, S Ono, H Murooka, Y |
| Keywords: | Astragalus sinicus; Mesorhizobium huakuii;Symbiotic plasmid |
| Issue Date: | Aug-2003 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Astragalus sinicus (Chinese Milk vetch), a green manure leguminous plant, harbors Mesorhizobium huakuii subsp. rengei strain B3 in the root nodules. The visualization of symbiotic plasmid of strain B3 showed the presence of one sym plasmid of about 425 kbp. Curing of sym plasmid by temperature and acrydine orange was studied. Growing rhizobial cells at high temperature (37°C) or treating the cells with acrydine orange at 50 mg/I eliminated sym plasmid of M. huakuii strain B3, which was confirmed by sym plasmid visualization and plant infection test of cured strains. |
| Page(s): | 912-914 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.41(08) [August 2003] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 41(8) 912-914.pdf | 702.24 kB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.