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Title: A protocol for shoot multiplication from foliar meristem of vanda spathulata (L.) Spreng
Authors: Decruse, S William
Gangaprasad, A
Seeni, S
Menon, V Sarojini
Keywords: Foliar meristem;Micropropagation;Orchid;Shoot multiplication;Vanda spathulata L.
Issue Date: Aug-2003
Publisher: NISCAIR-CSIR, India
Abstract: Leaf explants collected from flowering plants of vanda spathulata were cultured in Mitra medium with combinations of 6-benzyladenine (BA;13.2 -88.8 μM) and indole-3-acetic acid ( IAA; 0.0 - 85.6 μM). Combination of BA (66.6 μM) and IAA (28.5 μM) induced maximum shoots (17.33) from foliar meristems (leaf base). BA individually did not induce caulogenesis in leaf ex plants. For optimized multiplication, BA:IAA (2: 1μM) was essential at 22.2- 88.8 μM of BA. Recultured leaf explants produced lesser number of shoots compared to original explants and were nearly equal at combinations of 22.2-44.4 μM of BA and 5.7-28.5 μM of IAA. Rooting of shoots (>95%) occurred in medium containing banana pulp (75 gl-1) and IAA (5.7 μM) within 3-9 weeks. Plantlets with 2-5 roots of 2-5 cm length established easily in community pots at 80-90% rates without hardening.
Page(s): 924-927
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.41(08) [August 2003]

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