<span style="font-size:14.0pt;line-height: 115%;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#131313;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">A protocol for shoot multiplication from foliar meristem of <i>vanda spathulata</i> (L.) Spreng</span>

Abstract

<span style="font-size:14.0pt;line-height: 115%;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" color:#131313;mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:="" hi"="" lang="EN-IN">Leaf explants collected from flowering plants of <i>vanda spathulata</i> were cultured in Mitra medium with combinations of 6-benzyladenine (BA;13.2 -88.8 <i>μM</i>) and indole-3-acetic acid ( IAA; 0.0 - 85.6 <i>μM</i>). Combination of BA (66.6 <i>μM</i>) and IAA (28.5 <i>μM</i>) induced maximum shoots (17.33) from foliar meristems (leaf base). BA individually did not induce caulogenesis in leaf ex plants. For optimized multiplication, BA:IAA (2: 1<i>μM</i>) was essential at 22.2- 88.8 <i>μM</i> of BA. Recultured leaf explants produced lesser number of shoots compared to original explants and were nearly equal at combinations of 22.2-44.4 <i>μM </i>of BA and 5.7-28.5 <i>μM</i> of IAA. Rooting of shoots (>95%) occurred in medium containing banana pulp (75 gl^-1) and IAA (5.7 <i>μM)</i> within 3-9 weeks. Plantlets with 2-5 roots of 2-5 cm length established easily in community pots at 80-90% rates without hardening.</span>