Phylogenetic analysis of symbiotic and free-living cyanobacterial cultures using DNA amplification fingerprinting

Abstract

<span style="font-size:14.0pt;line-height: 115%;font-family:Aryan2Unicode;mso-fareast-font-family:" times="" new="" roman";="" mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">DNA amplification fingerprinting (DAF) of 17 cyanobacterial isolates belonging to symbiotic and free-living forms of 6 different genera was done. The dendrogram analysis of 17 cyanobacterial cultures revealed three major clusters. All <i>Westiellopsis </i>cultures formed the first major cluster and their nucleotide re latedness ranged between 71-93%. In the second major cluster, the symbiotic cyanobacterium <i>Anabaena </i>sp dominated and all <i>Anabaena </i>cultures showed 60 - 75% similarity with each other. <i>Nostoc muscorum </i>is related to <i>Allabaena variabilis </i>by 85% and formed the third major cluster. The dendrogram analysis of cyanobacterial isolates clearly revealed that free- living cyanobacterial cultures were closely related with each other and were diverse from the symbiotic forms.</span>