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Title: Isolation of etiological agent of hydropericardium syndrome in chicken embryo liver cell culture and its serological characterization
Authors: Kumar, Rajesh
Chandra, Rajesh
Shukla, S K
Keywords: Chicken embryo liver cell culture;Hydropericardium syndrome;Serological characterization
Issue Date: Aug-2003
Publisher: NISCAIR-CSIR, India
Abstract: The virus causing hydropericardium syndrome was isolated in chicken embryo liver (CEL) cell culture from livers obtained from naturally infected broilers. The cytopathic effects characterized by rounding and degeneration of cells were visible 36 hr post infection in first passage. At 4th passage level, the infectivity titre was 5.24 log1o TCID50/ml. In May-Grunwald and Giemsa stained cells, basophilic intranuclear inclusions (' bird eye' inclusion), typical of aviadenovirus infection, were observed. The specificity of inclusion was confirmed by indirect immunofluorescence. Various serological tests, such as agar gel precipitation test, counter immuno electrophoresis, micro serum neutralization test and enzyme linked immunosurbent assay were also standardized to confirm the isolation of etiological agent of hydropericardium syndrome in CEL cell culture and to diagnose the disease in poultry.
Page(s): 821-826
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.41(08) [August 2003]

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