Phytochemical investigation and evaluation of anti-inflammatory and anti-arthritic activities of essential oil of Strobilanthus ixiocephala Benth.

Abstract

Column chromatographic fractionation of essential oil obtained by hydrodistillation from the flowering tops of S.<i>ixiocephala </i>resulted in the isolation of β-<span style="font-size:14.0pt;line-height:115%;font-family:" times="" new="" roman";="" mso-fareast-font-family:hiddenhorzocr;color:black;mso-ansi-language:en-in;="" mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">caryophyllene<span style="font-size:14.0pt;line-height:115%;font-family:HiddenHorzOCR; mso-hansi-font-family:" times="" new="" roman";mso-bidi-font-family:hiddenhorzocr;="" color:black;mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:="" hi"="" lang="EN-IN">, <span style="font-size:14.0pt;line-height:115%; font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" color:black;mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:="" hi"="" lang="EN-IN">fenchyl acetate, T-cadinol and a new sesquiterpene alcohol for which a name ixiocephol has been proposed. The β-<span style="font-size: 14.0pt;line-height:115%;font-family:" times="" new="" roman";mso-fareast-font-family:="" hiddenhorzocr;color:black;mso-ansi-language:en-in;mso-fareast-language:en-in;="" mso-bidi-language:hi"="" lang="EN-IN">caryophyllene<span style="font-size: 14.0pt;line-height:115%;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";color:black;mso-ansi-language:en-in;mso-fareast-language:="" en-in;mso-bidi-language:hi"="" lang="EN-IN"> and fenchyl acetate were identified by Co-TLC with authentic samples whereas T-cadinol and ixiocephol were structurally elucidated by UV, IR,^{<span style="font-size:14.0pt;line-height: 115%;font-family:Arial;mso-fareast-font-family:" times="" new="" roman";color:black;="" mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">1</span>}<span style="font-size:14.0pt;line-height:115%;font-family:Arial; mso-fareast-font-family:" times="" new="" roman";color:black;mso-ansi-language:en-in;="" mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">H <span style="font-size:14.0pt;line-height:115%;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";color:black;mso-ansi-language:en-in;="" mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">NMR,¹³C NMR and Mass spectral data. The GC-MS analysis of the essential oil has also revealed the presence of various monoterpenoids and sesquiterpenoids. The essential oil of S. <i>ixiocephala </i>demonstrated a dose dependant anti-inflammatory activity in carrageenan-induced rat paw oedema. <span style="font-size:14.0pt;line-height: 115%;font-family:Arial;mso-fareast-font-family:" times="" new="" roman";color:black;="" mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">It <span style="font-size:14.0pt;line-height:115%;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";color:black;mso-ansi-language:en-in;="" mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">has also revealed good activity in cotton pellet granuloma and adjuvant induced arthritis model in rats. </span></span></span></span></span></span></span></span></span>