<i><span style="font-size:14.0pt;line-height: 115%;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#202020;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">In <span style="font-size:14.0pt;line-height:115%; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#444444;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">v<span style="font-size:14.0pt;line-height:115%; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#202020;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">itr<span style="font-size:14.0pt;line-height:115%; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#444444;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">o </span></span></span></span></i><span style="font-size:14.0pt;line-height:115%; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#202020;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">control of fa<span style="font-size:14.0pt;line-height: 115%;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#444444;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">sc<span style="font-size:14.0pt;line-height:115%; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#202020;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">iation in proliferating nucel1ar embryos of <i>Mangif</i><i><span style="font-size:14.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";color:#444444;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-IN">e<span style="font-size:14.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";color:#202020;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-IN">ra <span style="font-size:14.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";color:#323232;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-IN">indica </span></span></span></i><span style="font-size:14.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";color:#202020;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-IN">L.<span style="font-size:14.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";color:#323232;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-IN">var <span style="font-size:14.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";color:#202020;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-IN">totapari red <span style="font-size:14.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";color:#444444;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-IN">s<span style="font-size:14.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";color:#202020;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-IN">mall for cloning</span></span></span></span></span></span></span></span>

Abstract

Nucellar tissue contained in ovular halves of young fruits of <i>Man</i><i>g</i><i>if</i><i>e</i><i>r</i><i>a </i><i>indica </i>L. totapari red small, a dwarfing rootstock, differentiated fasciated embryonal structures in presence of 6-benzylaminopurine [BA P(0.15 mg l^-1)] 1, 6-(γ-γ-dimethylallylamino) purine [2iP(0.15 mg l^-¹)] and indole-3- acetic acid [(IAA(0.5 mg l^-¹)] incorporated in the semisolid medium during 50-60 days. Due to embryonal fasciation, hardly 2-3 well-formed embryos could be obtained per culture of proliferating embryos. Of the 3 ethylene inhibitors [ L-α-(2-aminoethoxyvinyl)-glycine-HCl (AVG), AgNO₃, and salicylic acid (SA)]used, embryonal fasciation and necrosis of intervening tissue was completely control led by 3-4 subcultures of fasciated mass of embryos under the influenee of AVG (0.05 mg l^‑1) in presence of adenine sulphate [AdS (50mg l^-¹)] incorporated in the same medium. Almost synchronized development of isolated embryos, measuring ca 2 cm in length, was observed in a different medium used in liquid stationary state and supplemented. particularly with stress-producing substances [abscisic acid (ABA 0.01 mgl^-¹) : and polyethylene glycol (PEG, 100 mg l^-¹) besides certain other modifications, About 34% convertibility of processed embryos was obtained during a period of 90 days. The plantlets had well -developed roots along with laterals which were longer than leafy shoots. In <i>vitro</i> raised plants survived <i>ex </i><i>vi</i><i>tro</i><i> </i>for about 2 months.