Enhancement of metastatic potential of mouse B-16-melanoma cells to lung after treatment with gangliosides of B-16-melanoma cells of higher metastatic potential to lung

Abstract

Mouse B16LuF1 melanoma cells of lower metastatic potential to lung were treated in <i>vitro </i>with same concentration (50<i>μM</i>) of gangliosides isolated from B16LuF5, B16LuF9 or B16LuF10 cells with higher metastatic potential to lung

<span style="font-size:14.0pt;line-height:115%;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";color:#767676;mso-ansi-language:en-in;="" mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">(LuF1< LuF5< LuF9< LuF10) and injected to groups of normal mice through tail vein. The number of metastatic tumor modules formed in lung increased in mice receiving B16LuF5, B16LuF9 und B16LuF10-ganglioside-treated B16LuF1 cells compared to mice receiving B16LuF1 cells without any ganglioside treatment. Metastatic potential of B 16LuF1 cells gradually increased after treatment with gangliosides of B16-melanoma cells of increasing metastatic potential to lung. The six major gangliosides isolated from B16LuF10 cells corresponded with standard gangliosides GT1b, GD1b, GD1a. GM1, GM2 and GM3 respectively on TLC-analysis. When B16LuF1 cells were treated in <i>vitro</i> with each of these six individual gangliosides and injected to groups of normal mice through tail vein the number of tumor nodules formed in lung varied. The four groups of mice receiving B16LuF1 cells treated with each of four gangliosides corresponding to GT1b, GD1b,GD1a or GM1 produced lung metastasis comparable to that of untreated control group. Only remaining two gangliosides high corresponded with standard gangliosides GM2 and GM3 increased metastatic potential of B16LuF1 cells. Thus, these results indicated that gangliosides GM2 and GM3 of Bl6-melanoma cells are definitely associated with metastatic potential of these tumor cells.</span>