Effect of estradiol- 17β on cell area, lumen area and trehalase activity of <span style="font-size:14.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";color:black;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-IN">posterior silk gland of <i>Bombyx mori</i> L.</span>

Abstract

<span style="font-size:14.0pt;line-height: 115%;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" color:black;mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:="" hi"="" lang="EN-IN">Estradiol- 17β (E₂) at the dose of 1μg/g caused an increase in cell area, lumen area and the total (cell <span style="font-size:14.0pt;line-height:115%;font-family:" times="" new="" roman";="" mso-fareast-font-family:fd1433539-identity-h;color:black;mso-ansi-language:="" en-in;mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">+ <span style="font-size:14.0pt;line-height:115%;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";color:black;mso-ansi-language:en-in;="" mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">lumen) area of posterior silk gland (PSG) in <i>Bombyx mori</i> indicating that exogenously applied estradiol- 17β has a regulatory influence on silk gland activity. A dose-dependent variation in trehalase activity of PSG was found on the 5th day after topical administration of estradiol on 1st and 2nd day of the fifth larval instar. Of all the doses of E₂used, 1μg/g dose had maximum stimulatory effect on trehalase activity. Co-administration of each of a specific receptor antagonist for estradiol, the ICI-182780 and a protein biosynthetic blocker, cycloheximide with E₂ suppressed the E₂-induced increase in silk gland activity. The results suggest some specific metabolic action of E₂ on silk gland and offer a promising way for future investigations regarding the physiological significance of vertebrate steroids in insects.</span></span></span>