Characterisation of sapindosides in <i>Sapindus mukorosii </i>saponin (reetha <span style="font-size:14.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";color:black;mso-ansi-language:EN-IN; mso-fareast-language:EN-IN;mso-bidi-language:HI" lang="EN-IN">saponin) and quantitative determination of sapindoside B*</span>

Abstract

<span style="font-size:14.0pt;line-height: 115%;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" color:black;mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:="" hi"="" lang="EN-IN">High performance thin layer chromatography (HPTLC) method as well as high performance liquid chromatographic (HPLC) method combined with ES-MS are developed and validated for fingerprinting (profiling) <i>Sapindus saponin</i> and quantitative determination of Sapindoside B in bulk drug samples of <i>Sapindus saponin </i>and its formulation Consap cream. The separation of saponins using TLC is achieved on precoated silica gel plates using chloroform: methanol: water as mobile phase. Detection of the spots is done at the 630 nm. The identification of saponins is done by LC-MS, using Electro Spray Ionisation [ESI] technique. The HPLC method involves chromatography of the saponins on reverse phase (C-18) column, using acetonitrile-water gradient as mobile phase. The detection is done by UV -visible detector at a wavelength of 215 nm. Calibration graphs are found to be linear over the range 11-220 <span style="font-size:14.0pt;line-height:115%;font-family:" times="" new="" roman";="" mso-fareast-font-family:hiddenhorzocr;color:black;mso-ansi-language:en-in;="" mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">μg <span style="font-size:14.0pt;line-height:115%;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";color:black;mso-ansi-language:en-in;="" mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">in HPTLC and 30-200 <span style="font-size:14.0pt;line-height:115%;font-family:" times="" new="" roman";="" mso-fareast-font-family:hiddenhorzocr;color:black;mso-ansi-language:en-in;="" mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">μg/mL <span style="font-size:14.0pt;line-height:115%;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";color:black;mso-ansi-language:en-in;="" mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">in HPLC method. The methods developed are being used for the analysis of the bulk drug samples and Consap cream samples.</span></span></span></span></span>