Modified Cold Percolation Method for Extracting Oil from Oil Seeds

Abstract

Oil extraction by different methods like soxhlet, automated soxhlet, sonication, microwave, Super Critical Fluid Extraction (SFE), Accelerated Solvent Extraction (ASE) consume 15 to 500 mL of solvent and average extraction time per sample varies from 12 min to 48 h. Such methods are inconvenient to follow, where a large number of small sized samples are to be processed, in order to detect the small effects of enzymatic treatment. For this purpose, a simplified cold percolation method is quite effective and suitable. However, the extraction period is too small (2 to 3 min) to allow manipulation of oil extraction quantities and rates. In this paper, we present a modified cold percolation method for regulating extraction of oil from oil seeds, particularly suitable for enzymatic studies.