Optimization of Cellulase Production by <i>Trichoderma reesei </i>ATCC 26921 Using a Simplified Medium on Water Hyacinth Biomass

Abstract

<span style="font-size:14.0pt;line-height: 115%;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" color:black;mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:="" hi"="" lang="EN-IN">Some environmental (pH and temperature) and biochemical (substrate concentration, nitrogen source and other nutrients) factors influencing cellulase production by <i>Trichoderma reesei </i>ATCC 26921 were studied during its growth in Mandels and Andreotti medium containing water hyacinth biomass as the major carbon source. Optimum pH, temperature and substrate concentration were found to be 4.8, 31 ± 1°C and 4 per cent (w/v) milled water hyacinth, respectively, for the maximum production of FPase, CMcase, and β-glucosidase. Among the different nitrogen sources tested, ammonium sulphate was found to be the best for cellulase production. In an attempt to simplify the composition of the basal medium, all other nutrients except (NH₄)₂SO₄ , KH₂P O₄ , yeast-extract and Tween-80 were omitted from the medium. This did not reduce much the yield of cellulase. Addition of poultry manure further increased the cellulase production.</span>