Kinetics and binding studies on the reaction of cyanopropyl(aquo)cobaloxime with CT-DNA and amines

Abstract

The ligand substitution reactions of cyanopropyl (aquo) cobaloxime have been studied with various amines such as Methylamine, ethylamine, butylamine, pentylamine, hexylamine, cyclopropylamine, cyclohexylamine and cyclope ntylamine, as entering nucleophiles. The entering nucleophile displaces the

molecule <i>trans </i>to the cyanopropyl group to form the six-coordinated

complex, [CNCH₂CH₂ CH₂Co(DH)₂L]. The rate constants have been determined by varying the <i>p</i>H and concentration of the ligand under pseudo first order conditions. Equilibrium constants have been determined as a function of <i>p</i>H, for the binding of amines to [CNCH₂CH₂ CH₂Co(DH)₂L]. Study

shows that the entering nucleophile participates in the transition state, and hence, SN¹ mechanism is proposed. Antimicrobial activity of cyanopropyl (aquo)cobaloxime on <i>E</i> <i>coli </i>has been studied. The interaction of cyanopropyl(aquo) cobaloxime with CT DNA has been studied spectrophotometrically and binding constant has been calculated.