Micropropagation of Terminalia bellerica Roxb. from juvenile explants

Abstract

An in vitro micropropagation system has been developed for Terminalia bellerica Roxb., an important Indian medicinal plant. Nodal segments obtained from 15-d-old aseptically grown seedlings were used as explants. MS medium containing 1.5 mg L⁻¹ BAP was found most suitable for culture initiation. Although shoot multiplication was achieved on MS medium containing BAP and Kn, the maximum number of shoots was obtained with 1.5 mg L⁻¹ BAP. Best rooting response (60%) was observed on medium containing quarter strength MS salts, 0.6% agar and 0.1 mg L⁻¹ IBA. Plantlets were hardened initially in culture room conditions and then transferred to misthouse.