Polymorphism in DRB3 exon 2 by PCR-RFLP and its association with mastitis in Nili-Ravi breed

Abstract

The present investigation was undertaken to study the genetic polymorphism in exon 2 of DRB3 gene in Nili-Ravi (n=25) buffalo breed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and its association with mastitis. The gDNA was isolated from whole blood samples. When 304 bp PCR product of exon 2 of DRB3 gene was digested with Rsa1, 11 genotypes, viz., b/b, c/c, f/f, o/o, s/s, f/o, b/f, b/o, o/s, b/l and l/s, with frequency range 0.04-0.16 and 6 alleles, viz., b, c, f, l, o and s with frequency range 0.08-0.26 were observed. HaeIII detected 6 genotypes, viz., a/a, e/e, d/d, a/b, b/d and b/e with frequency range 0.04-0.28 and four alleles, viz., a, b, d and e with frequency range 0.08-0.6. However, Pst1 revealed 5 genotypes, viz., y/y, z/z, x/y, x/z, s/z and y/z with frequency range 0.08-0.32 and four alleles, viz., x, y, z, and s with frequency range 0.04-0.42. These results revealed that exon 2 of DRB3 gene was highly polymorphic in Nili-Ravi breed. Certain genotypes (c/c, f/l, b/f, o/o, l/s, a/a and e/e) were observed only in healthy animals, while others (b/f, b/o, f/o and y/z) in mastitis cases. Result to be tested on large sample size before its practical application.