Separation of amino acids on alumina layer developed with oil-in-water microemulsion

Abstract

Oil-in-water microemulsion systems have been used as mobile phase in thin layer chromatographic separation of aliphatic and aromatic amino acids on plain (or non-impregnated) alumina and Li⁺, Na⁺ or NH₄⁺ - impregnated alumina layers. The results

obtained with water and oil-in-water microemulsions have been compared. The lower limits of detection of amino acids have also been determined. Proline, dl-valine and dl- aspartic acid have been selectively separated from other amino acids.