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http://nopr.niscpr.res.in/handle/123456789/18639| Title: | Study on the flow injection method for the determination of L-cysteine with a Cu(II) complex by fluorescence quenching |
| Authors: | Cao, Qiu-E Zhao, Yunkun Hu, Zhide Xu, Qiheng |
| Issue Date: | Dec-2001 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A flow injection method for fluorescence determination of cysteine using 5-(3-fluo-4-chloropheny lazo)-8-benzenesulfonamidoquinoline (FCPBSQ), a new fluorescence reagent, has been proposed. The method is based on the quenching of fluorescence in the Cu(II)-FCPBSQ system by cysteine in nearly neutral medium. Under the optimum conditions, the fluorescence quenching intensity increases linearly with increase in the concentration of cysteine in the range of 0.050-6.0 μg/ml. The detection limits of this method is 0.010 μg/ml with a sample frequency of 64h-1. The method is free of interference from several metal ions and amino acids without -SH group and has been successfully applied for the determination of cysteine in the pharmachemical samples. The mechanism of the fluorescence quenching is also discussed. |
| Page(s): | 1344-1347 |
| ISSN: | 0975-0975(Online); 0376-4710(Print) |
| Appears in Collections: | IJC-A Vol.40A(12) [December 2001] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJCA 40A(12) 1344-1347.pdf | 809.47 kB | Adobe PDF | View/Open |
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