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http://nopr.niscpr.res.in/handle/123456789/18675| Title: | Fungal chitinase production and its application in biowaste management |
| Authors: | Narayanan, K. Chopade, Nagesh Raj, P. Vasanth Subrahmanyam, V.M. Rao, J.Venkata |
| Keywords: | Chitinase;Optimization;Aspergillus terreus;Chitin source;Biowaste management |
| Issue Date: | Jun-2013 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Chitinases find wide applications in pest control, pollution abatement and in management of marine wastes. A marine soil isolate Aspergillus terreus, having chitinase activity was cultivated on sucrose-peptone-yeast extract medium. Colloidal chitin replaced sucrose in the production medium. The cultivation conditions were optimized using single parameter approach and Placket-Burman statistical design. The enzyme activity was estimated using di-nitrosalicylic acid method. Chitinolytic activity was 52 U/ml in shake flask experiments and 29.2 U/ml on a 3 L laboratory fermenter. The enzyme extracted with cold acetone showed a prominent band having a molecular weight of 52.8 kDa on SDS PAGE. The organism utilized chitin present in mushroom (Agaricus bisporus) when used as a sole source of carbon with the enzyme activity being 82.38 U/ml. The enzyme was stable at wide temperature range 30- 70 °C maximum activity seen at 50 °C. The study has a considerable significance and impact in bioremediation of biological waste. |
| Page(s): | 393-399 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Appears in Collections: | JSIR Vol.72(06) [June 2013] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| JSIR 72(6) 393-399.pdf | 151.42 kB | Adobe PDF | View/Open |
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