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http://nopr.niscpr.res.in/handle/123456789/18979| Title: | Comparative evaluation of sensitivity of RNA-polyacrylamide gel electrophoresis and dot immunobinding assay for detection of bluetongue virus in cell culture |
| Authors: | Prasad, G Minakshi |
| Issue Date: | Feb-1999 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Bluetongue virus serotype 1 (Avikanagar isolate) was grown in BHK-21 cell line and titrated. The titre of the virus in BHK-21 cell line was 106 TCID50/ml . RNA-polyacrylamide gel electrophoresis (RNA-PAGE) and dot immunobinding assay (DIA) were performed on to-fold serial dilutions of the sonicated cell culture material. The results indicated that the minimum limit of detection of the virus by RNA- PAGE and DIA was 105 TCID50/ml. |
| Page(s): | 157-160 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.37(02) [February 1999] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 37(2) 157-160.pdf | 2.11 MB | Adobe PDF | View/Open |
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