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Title: Immunomodulation of macrophages by radio-detoxified lipopolysaccharide of Salmonella typhimurium
Authors: Naidu, Mamta D
Chander, Ramesh
Issue Date: Mar-1999
Publisher: NISCAIR-CSIR, India
Abstract: Lipopolysaccharide (LPS) and radio-detoxified LPS (Rd-LPS) from Salmonella typhimurium were analysed for their ability to stimulate murine peritoneal exudate cells (PEC) and macrophages. Rd-LPS induced much more inflammatory response as compared to LPS. PEC numbers/mouse obtained were significantly higher (3-fold) in response to Rd-LPS than LPS. The haemorrhage was induced in mice by LPS but not by Rd-LPS. Activation of macrophages ill vivo by Rd-LPS was significantly higher as compared to LPS. This was evident from the increase levels of their lysosomal enzymes and cytokines. Rd-LPS induced 10-fold increase in acid phosphatase contents of macrophages as compared to controls while only 7-fold increase was obtained with LPS. Arylsulfatase and β-glucuronidase increased by about 2-fold by Rd-LPS and LPS. Macrophages incubated with Rd-LPS in vitro showed 16-fold and 20-fold increase in the cell associated levels of arylsulfatase and β-glucuronidase respectively as compared to unstimulated cells. On the other hand , only 6-fold increase was observed in response to LPS in the levels of both the enzymes. TNF- and IL-1 secreted by macrophages increased considerably in response to Rd-LPS as compared to those released by LPS. Rd-LPS, thus seems to be a better immunomodulator than untreated LPS.
Page(s): 283-289
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.37(03) [March 1999]

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