Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/19266| Title: | In vitro propagation of Aerva lanata (L.) Juss. ex Schult. through organogenesis |
| Authors: | Sahu, Alok Ranjan Rath, Sobha Chandra Panigrahi, Jogeswar |
| Keywords: | In vitro;Nodal explant;Medicinal herb |
| Issue Date: | Apr-2013 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In
vitro plantlet regeneration in Aerva lanata (L.)
has been achieved from nodal segments cultured on MS medium supplemented with
growth regulators. Nodal explants from healthy field grown plants were cultured
on MS medium fortified with various concentrations of BA (N6-benzyladenine), KN (kinetin) and NAA ( -napthalene acetic acid) either alone or in combinations. The combination
of BA and KN (3.0 mg/L each) was the best for multiple shoot induction through
direct organogenesis. While addition of NAA (1.0 or 1.5 mg/L) to the optimal
combination of BA and KN in the medium was the best and produced the maximum (10.66±1.15)
multiple shoots via callus mediated
organogenesis. Well-developed shoots (>3cm) were successfully rooted on half
strength MS medium containing IBA (indole butyric acid; 0.5 mg/L). The
regenerated plantlets were successfully established in soil with survival rate
of 72±4%. The protocol described is simple,
rapid and efficient for in vitro propagation of A. lanata from
nodal explants.
|
| Page(s): | 260-264 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.12(2) [April 2013] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 12(2) 260-264.pdf | 143.48 kB | Adobe PDF | View/Open |
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