Regeneration of plantlets from nodal explant-derived callus cultures of <i style="mso-bidi-font-style:normal">Albizia lucida</i> Benth.

Abstract

<i style="mso-bidi-font-style:normal">Albizia lucida</i> Benth. is a forest tree known for timber, gum and tannin, and lac production. Micropropagation of <i style="mso-bidi-font-style:normal">A. lucida</i> can potentially address large-scale production of genetically identical superior genotypes. In the present study, a callus-mediated regeneration protocol has been established from nodal explants of <i style="mso-bidi-font-style:normal">A. lucida</i> for micropropagation. Murashige and Skoog’s (MS) medium supplemented with plant growth regulators like <span style="mso-fareast-font-family:Calibri; mso-ansi-language:EN-IN">thidiazuron (TDZ; 0.5 µ<i style="mso-bidi-font-style:normal">M</i>) and <span style="mso-fareast-font-family: Calibri;mso-ansi-language:EN-IN">6-benzylaminopurine (BAP; 2.22 m<i style="mso-bidi-font-style:normal">M</i>) were found efficient in callus induction and multiplication. From the multiplied greenish and compact organogenic calli, shoots were regenerated best on MS media consisting BAP (8.88 m<i style="mso-bidi-font-style:normal">M</i>), NAA (1-napthaleneacetic acid; 0.54 m<i style="mso-bidi-font-style:normal">M</i> ) and AgNO₃ (5.89 m<i style="mso-bidi-font-style:normal">M</i>). The excised microshoots were rooted efficiently on ½ MS medium supplemented with IBA (indole butyric acid; 9.8 m<i style="mso-bidi-font-style: normal">M</i>) and BAP (0.11 m<i style="mso-bidi-font-style: normal">M</i>). The rooted shoots were acclimatized in polybags containing garden soil, sand and FYM (1:1:1) and then transferred into the open. About 66.67% of the plants survived in the end. The genetic fidelity of the regenerated plantlets was found identical to the mother plant using SRAP primers.

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