Entrapment of Sorghum root oxalate oxidase into polyvinyl alcohol membrane

Abstract

A Cl^- and NO₃^- insensitive oxalate oxidase, purified from the roots of 10-day old seedlings of grain Sorghum has been immobilized on polyvinyl alcohol (PVA) membrane through entrapment with 96.07% retention of initial activity. The membrane bound enzyme showed an increase in optimum <i>p</i>H<i> </i>(from 5.0 to 6.5), time of incubation (from 5 to 10 min) and

<i>K</i>_m<i> </i>for oxalate (from 0.38 to 6.23 m<i>M</i>)<i>, </i>but decrease in incubation temperature for maximum activity (from 37 to 30°C) and <i>V</i>_max (from 70 nmol/min/ml to 9.7 nmol H₂O₂/min) and was unaffected by Cl^- and NO₃^-. The membrane bound enzyme lost 50% of its initial activity after 30 days of storage at room temperature. The use of membrane bound oxalate oxidase in

determination of serum oxalate of urinary stone patients is demonstrated.