A new form of Scatchard plot to study binding of fluoride ion on urease by isothermal titration calorimetry

Abstract

A new equation with a useful simple graphical method, very similar to the Scatchard plot was introduced to obtain the equilibrium constant and the enthalpy of binding using isothermal titration calorimetric data. On applying this simple graphical method to study the binding of fluoride ion on jack bean urease, the dissociation equilibrium constant obtained was remarkably consistent with the inhibition constant obtained from assay of enzyme activity in the presence of fluoride ion.