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Title: A Modified Spectrophotometric Assay of Superoxide Dismutase
Authors: Kakkar, Poonam
Das, Ballabh
Viswanathan, P N
Issue Date: Apr-1984
Publisher: NISCAIR-CSIR, India
Abstract: A simple and rapid method for the assay of superoxide dismutase in biological samples is described. Present method takes advantage of the inhibition of NADH-dependent-nitroblue tetrazolium reduction by the dismutase. Inhibition of the chromogen formation by superoxide dismutase was linear with increase in enzyme concentrations. The chromogen extract in butanol was stable even up to 48 hr. Superoxide dismutase activity, as assayed by the modified method, was in good agreement with that obtained using other methods.
Page(s): 130-132
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.21(2) [April 1984]

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