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http://nopr.niscpr.res.in/handle/123456789/20367| Title: | Rapid synthetic access to caged RNA |
| Authors: | Rodrigues-Correia, Alexandre Stoess, Tatjana Wilhelm, Julia Berlin, Beata Heckel, Alexander |
| Keywords: | RNA;Caged RNA;Light-activatable RNA;Duplex destabilizing |
| Issue Date: | Aug-2013 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Light-activatable RNA with “caged” nucleobases is a versatile tool for light-control of RNA interference, riboswitch folding or miRNA or ribozyme action. Till date the respective caged and protected phosphoramidites for the solid-phase RNA synthesis are obtained in lengthy syntheses. Herein we explore the use of convertible nucleoside residues for a rapid access to nucleobase-caged RNA. In these studies we have also prepared the first RNA with a caging group on an N2-position of a guanosine. The obtained caged sequences are compared in their duplex destabilizing ability via melting temperature measurements. |
| Page(s): | 1014-1018 |
| ISSN: | 0975-0975(Online); 0376-4710(Print) |
| Appears in Collections: | IJC-A Vol.52A(08-09) [August-September 2013] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJCA 52A(8-9) 1014-1018.pdf | 90.25 kB | Adobe PDF | View/Open |
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