Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/20369| Title: | Fabrication of biotin functionalised SiO2 EM grid for studying biotin tagged biomolecules |
| Authors: | Chakraborty, Indrani Saha, Abhijit Ghosh, Surajit |
| Keywords: | Electron microscopy;Surface functionalisation;Silica EM grid;Gold nanoparticles;Nanoparticles;Microtubules;Biotin |
| Issue Date: | Aug-2013 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A biotin functionalised SiO2 electron microscope grid has been developed for studying various biotin/avidin tagged biomolecules onto the grid surface under electron microscope. As an application, we have shown successful immobilisation of streptavidin gold nanoparticles and biotinylated microtubules through biotin-streptavidin-biotin interaction. This grid will be useful for studying the structures of various biotin-tagged proteins and their complexes under electron microscope. |
| Page(s): | 1026-1030 |
| ISSN: | 0975-0975(Online); 0376-4710(Print) |
| Appears in Collections: | IJC-A Vol.52A(08-09) [August-September 2013] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJCA 52A(8-9) 1026-1030.pdf | 145.94 kB | Adobe PDF | View/Open |
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