Regeneration and transformation studies in <i>Terminalia chebula </i>Retz.

Abstract

This study presents <i>in vitro </i>regeneration of <i>Terminalia chebula </i>Retz. to obtain complete plantlets from juvenile explants (hypocotyl and cotyledon). Dried seeds were inoculated on MS basal medium after surface sterilization with Bavistin (0.2%)

alone and followed by HgCl₂ (0.1%), resulted in maximum (75%) germination. Hypocotyl showed 90% and cotyledon 75% callus induction on MS basal medium containing 1.0 mg/l 2, 4-D after 30 days of inoculation. Shoot regeneration was recorded only from cotyledonary callus on shoot induction medium comprising 1.5 mg/l BAP with 0.10 mg/l NAA with maximum 36.67% shoot regeneration. Maximum (43.75%) rooting was reported in ½ strength MS medium with 0.5% activated charcoal. Transgenic callus was produced through <i>Agrobacterium tumefaciens </i>mediated genetic transformation carrying <i>gus </i>and <i>npt-II </i>gene from cotyledonary

explants. Co-cultivation (72 h) preceded by pre-conditioning (72 h) was found best for callus induction. Successful integration of <i>gus </i>gene was reported.