A simple method for selective removal of human DNA from oral microbiome for functional metagenomic applications

Hemalatha, Devaraj; Rajendhran, Jeyaprakash; Gunasekaran, Paramasamy

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Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/20949

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DC Field	Value	Language
dc.contributor.author	Hemalatha, Devaraj	-
dc.contributor.author	Rajendhran, Jeyaprakash	-
dc.contributor.author	Gunasekaran, Paramasamy	-
dc.date.accessioned	2013-09-04T09:05:49Z	-
dc.date.available	2013-09-04T09:05:49Z	-
dc.date.issued	2013-09	-
dc.identifier.issn	0975-1084 (Online); 0022-4456 (Print)	-
dc.identifier.uri	http://hdl.handle.net/123456789/20949	-
dc.description	572-576	en_US
dc.description.abstract	This study presents a simple filtration based technique to reduce human DNA contamination in mouthwash samples. Quantitative PCR evaluated efficiency of proposed method in removal of human DNA and selective extraction of bacterial DNA. The b-globin and 16S rDNA were targeted to measure the level of human and bacterial DNA respectively. When compared to unfiltered metagenomic DNA, there was 89% reduction in human DNA along with proportionate enrichment of bacterial DNA was obtained in filtered DNA. This strategy can serve as an efficient method for removal of human DNA and enrichment of microbial DNA in mouth wash samples usefull for functional metagenomic applications.	en_US
dc.language.iso	en_US	en_US
dc.publisher	NISCAIR-CSIR, India	en_US
dc.rights	CC Attribution-Noncommercial-No Derivative Works 2.5 India	en_US
dc.source	JSIR Vol.72(09-10) [September-October 2013]	en_US
dc.subject	Filtration	en_US
dc.subject	Oral metagenome	en_US
dc.subject	Real time PCR	en_US
dc.subject	Quantification	en_US
dc.title	A simple method for selective removal of human DNA from oral microbiome for functional metagenomic applications	en_US
dc.type	Article	en_US
Appears in Collections:	JSIR Vol.72(09-10) [September-October 2013]

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