Development of a microbial consortium for production of blend of enzymes for hydrolysis of agricultural wastes into sugars

Abstract

This study presents development of a blend of enzymes capable of degrading lignocellulosic biomass from a locally isolated microbial consortium through solid state fermentation. Different agro wastes (wheat bran, rice husk and pine needles) were taken in all combinations as a substrate and at 1:1:1 ratio, optimum production of blend of enzymes was obtained with 0.673 U/ml, 0.214 U/ml and 0.032 U/ml activities respectively after 3 days of incubation at 37°C. This blend of enzyme hydrolysed pine waste and released 0.454 g/g of sugar at 37°C at 120 rpm for 48 h. Produced blend of enzymes was found very potent and can have great application in biofuel, phytochemical and other industries.