Interaction of Ni(II) with nucleotides and dipeptides - A quantitative study

Abstract

Interaction of Ni (II) with purine nucleotides (5'-guanosine monophosphate and 5'-inosine monophosphate) and dipeptides (DL-alanyl-alanine and DL-alanyl-phenylalanine) in a 1:1 and 1:1:1 ratios has been investigated by potentiometric <i>p</i>H measurements at 25, 35 and 45°C. The formation constants for various systems are determined by computer program.

The stabilization of these complexes is expressed in terms of Δlog <i>K. </i>The stacking interaction associated with nucleotides and dipeptides have been identified with parameters like ΔΔ log <i>K, K</i>₁<i>, </i>% of (MLA)_st and ΔG⁰_s. The <i>p</i>H profiles of various species are generated in order to identify the stable species. The thermodynamic parameters associated with the above systems are also evaluated for a more comprehensive understanding of these reactions in solution.