Isolation and characterization of pectic polysaccharides from the fruits of <i>Naringi crenulata</i>

Abstract

Pectic polysaccharides have been isolated from the fruits of <i>Naringi crenulata </i>by extraction with water. The water extract contains large amount of protein. The polymers present in the

water extract are fractionated by graded precipitation with ethanol, anion exchange chromatography, and size exclusion chromatography. Characterization or the subtractions obtained therefrom by various chemical and physico-chemical methods of analysis reveals that the water extract contain pectic polymers substituted to various degrees with side chains comprising mainly or terminal, 1,4-, 1,6-, 1,3,6-linked galactose, together with lesser

amounts of 1,2,4- and 1,3-linked galactose residues. Arabinose residues are terminal, 1, 5-, 1,3,5-linked. These polymers contain acetyl groups and give viscous solution in water.