Micropropagation of <i style="mso-bidi-font-style: normal">Stemona tuberosa</i> Lour.—An endangered and rare medicinal plant in Eastern Ghats of India

Abstract

An <i style="mso-bidi-font-style:normal">in vitro</i> technique of multiple plantlet regeneration was developed for conservation of endangered wild medicinal plant <i style="mso-bidi-font-style:normal">Stemona tuberosa</i> Lour.. Murashige and Skoog (MS) medium supplemented with 7.0 mg/L Kn (kinetin) was found to be the optimum for axillary bud proliferation and multiple shoot induction. Excision and culture of nodal segments from the <i style="mso-bidi-font-style:normal">in vitro</i>-shoots on medium containing 7.0 mg/L Kn and 4.0 mg/L TDZ (thidiazuron) showed maximum number of shoot multiplication with 7.10<span style="font-family:Symbol;mso-ascii-font-family:" times="" new="" roman";="" mso-hansi-font-family:"times="" roman";mso-char-type:symbol;mso-symbol-font-family:="" symbol"="" lang="EN-GB">±0.37 shoots/node. Rapid shoot growth with simultaneous tuberous root formation was also observed in the same concentration. Shoots developed were rooted best on ½ strength MS with 1.0 mg/L IAA (indole acetic acid). <span style="mso-bidi-font-family:Times-Roman" lang="EN-GB">Plantlets established in pots exhibited 85% survival. Plantlets successfully established in field exhibited morphological characters identical to mother plants.

</span></span>