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http://nopr.niscpr.res.in/handle/123456789/21857| Title: | Effect of glucose and nitrogen source on caffeine degradation by four filamentous fungi |
| Authors: | Pai, Pooja V Pai, Ashwini Pai, Supriya Devadiga, Sushma Y Nayak, Vibha Rao, C Vaman |
| Keywords: | Caffeine degradation;Glucose;HPLC;Nitrogen |
| Issue Date: | Jul-2013 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A time bound study of caffeine degradation by fungi, viz., Chrysosporium keratinophilum, Gliocladium roseum, Fusarium solani and Aspergillus restrictus, isolated from the coffee pulp was undertaken. The degradation was studied at 1 g/L concentration of caffeine with 1-8 g/L glucose, and with or without nitrogen (NH4Cl) source at 2 g/L in minimal liquid medium by subjecting to shake flask culture at 120 rpm and 30°C. The caffeine degradation was analyzed up to 7 d at 24 h interval by HPLC. It is evident from the present study that varying concentrations of glucose did not affect the caffeine degradation by all the four test fungi. Over all, C. keratinophilum showed significantly higher rate of caffeine degradation compared to other test fungi, irrespective of presence or absence of nitrogen source in the medium. However, the caffeine degradation by C. keratinophilum was on the higher side in the absence of nitrogen source. In contrast, G. roseum, F. solani and A. restrictus showed better rate of caffeine degradation in the presence of nitrogen source in the medium. |
| Page(s): | 432-434 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.12(3) [July 2013] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 12(3) 432-434.pdf | 80.77 kB | Adobe PDF | View/Open |
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