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http://nopr.niscpr.res.in/handle/123456789/21864| Title: | Application of Plackett–Burman experimental design and Box and Wilson design to improve broad-spectrum antimicrobial compound |
| Authors: | Sharma, Deepika Manhas, Rajesh Kumari |
| Keywords: | Antimicrobial activity;MBC;MIC;PBD;RSM |
| Issue Date: | Jul-2013 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The present study is aimed at optimization of process parameters for production of antimicrobial compound from a potent antibiotic producing Streptomyces isolate. Among a total of 130 actinomycetes, isolate 2A showed antimicrobial activity against all the test organisms including drug resistant bacteria. 16S rRNA gene sequence analysis showed that strain 2A belongs to the genus Streptomyces and exhibited highest 16S rRNA gene sequence similarity (99.8%) with Streptomyces flavotricini NBRC12823T (AB184173). Comparison of physiological characteristics and DNA-DNA hybridization (manifested 36.7% similarity) with S. flavotricini suggests that 2A is a novel strain (GenBank acc. no. GQ906975). The traditional one-variable-at-a-time optimization strategy and statistically based experimental designs, Plackett-Burman design (PBD) and Box and Wilson design, were applied to provide an efficient approach for optimization. Statistical analysis using PBD demonstrated that KNO3, K2HPO4 and NaCl had significant positive influences on the production. In optimized medium, antimicrobial compound production was increased by 1.5-fold as compared to the basal production medium. In addition, the optimization enhanced the antimicrobial activity spectrum. The crude extract showed ability to inhibit all tested bacteria with MIC ranging from 15.6-125 μg mL‐1 and MBC from 31.2-500 μg mL‐1. |
| Page(s): | 386-394 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.12(3) [July 2013] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 12(3) 386-394.pdf | 624.27 kB | Adobe PDF | View/Open |
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