Development of quality control markers for <i>Ulmus wallichiana</i> Planchon: An Indian traditional plant for osteogenic activity

Abstract

<i><span style="mso-ansi-language:EN-US" lang="EN-US">Ulmus wallichiana</span></i><span style="mso-ansi-language:EN-US" lang="EN-US"> Planchon, is an Indian folk traditional plant used for the treatment of fractured bones in folklore tradition of Uttarakhand Himalaya, India. During chemical investigations, three major compounds, Ulmoside A &amp; B, and Naringenin-6-C-β-D glucopyranosideis for osteogenic activity have been isolated from bark of this plant species. This paper presents macro and microscopic study, physicochemical parameters and chemo-profiling of stem bark of <i>U. wallichiana</i> through Q TOF HRMS for development of quality control markers and identification of crude samples. Transverse section (TS) stem bark and powder microscopy shows crushed rhytidoma consisting plenty of mucilaginous canals followed by crushed cork, cells of the cork filled with dark brown content. Whereas, authenticity and quality of raw materials can be determinate with exact calculated mass value of marker compounds for osteogenic activity at 467, 451 and 435 using Q TOF HRMS technique. Ursolic acid, β-sitosterol and lupeol at R_f value 0.25, 0.36 and 0.44 has also identified as HPTLC marker for identification and authentication of crude drug samples of stem bark of <i>U. wallichiana</i>.

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