Lipase immobilized on walls of plastic beaker: Kinetic properties and application in washing of oil stained cloth

Abstract

Commercial porcine pancreas lipase was immobilized covalently onto alkylamine glass-beads affixed on the inner wall of a plastic beaker by an adhesive. The immobilized enzyme retained 10.8% of the initial activity of free enzyme with a conjugation yield of 52 mg/g support. The optimum pH and incubation temperature were decreased, while time for linearity and <i>K</i>_m<i> </i>for triolein of enzyme were increased after immobilization. The utility of immobilized enzymes in removal of oil stain from cotton cloth by various detergents was tested by chemical method. All the detergents gave better washing in presence of immobilized lipase than that by detergent alone. Further, the washing by cheaper (non-enzymic) detergents in presence of immobilized lipase was almost similar to that by expensive (enzymic) detergents. The immobilized enzyme was used about 100 times without any considerable loss of activity.