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http://nopr.niscpr.res.in/handle/123456789/23051| Title: | Isolation of poly (A+) mRNA for downstream reactions from some medicinal and aromatic plants† |
| Authors: | Shukla, Ashutosh K Shasany, Ajit K Khanuja, Suman P S |
| Keywords: | Aromatic plant;Catharanthus roseus;Medicinal plant;Poly (A+) mRNA;RNA isolation |
| Issue Date: | Feb-2005 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In the present protocol for extraction of RNA, hexadecyltrimethylammoniumbromide (CTAB) and insoluble polyvinylpyrrolidone were used followed by LiCl precipitation, CsCl ultracentrifugation and finally poly (A+) mRNA was isolated with the help of oligo(dT)-cellulose columns. The isolated poly (A+) mRNA was found to be suitable for cDNA-AFLP and suppression subtractive hybridization applications. It is a modified and consolidated protocol based on previously described methods for isolated steps and works better for medicinal and aromatic plants. High yield of poly (A+) mRNA coupled with its amenability for downstream reactions like RT-PCR. northern blotting and cDNA synthesis for library construction is a key feature of the present protocol. |
| Page(s): | 197-201 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.43(02) [February 2005] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 43(2) 197-201.pdf | 1.28 MB | Adobe PDF | View/Open |
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